Services Provided by Rodent Histopathology Core


Technical Contact:  
Li Zhang : 617-432-2409

Core Location:
Harvard Medical School
220 Longwood Ave. Goldenson 126 Tax ID #04-2103580




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Histopathology Services
  Conventional Histology Services
   Necropsy [Gross exam/harvest only. Does not include cassetting, processing, embedding, & sectioning]
   Tissue Trimming and Cassetting - Paraffin
   Processing- Paraffin
   Embedding- Paraffin
   Creation of Frozen Block in OCT
   H&E Stain [Paraffin & Frozen]
   Dehydrate & Coverslip Only
   Paraffin Sectioning- Per Slide
   Paraffin Sectioning- Per Section
   Frozen Sectioning- Per Slide
   Frozen Sectioning- Per Section
   Frozen Sectioning w/ Tape- Per Slide
   Paraffin Sectioning for Histogel- Per Slide
 
  Histological Staining - Bacteria, Fungi, and Inclusion Bodies
   Ziehl Neelsen Method for Acid Fast Bacteria [Class I]
   Grocott's Method for Fungi [GMS] [Class IV]
   Brown and Brenn Method for Gram +/ Bacteria [Class III]
 
  Histological Staining - Carbohydrate and Mucoproteins
   Congo Red for Amyloid [Class II]
   Crystal Violet for Amyloids [Class II]
   Periodic AcidSchiff's [PAS] [Class I]
   Mayer's Mucicarmine Method for Mucin and Cryptococcus [Class II]
   Alcian Blue for Mucosubstances [Class I]
 
  Histological Staining - Connective Tissue and Muscle
   Van Gieson's Method for Collagen Fibers [Class I]
   Mallory's Aniline Blue Collagen Stain [Class I]
   Gomoris Aldehyde Fuchsin Method [Class II]
   Wilder's Method for Reticulum [Class III]
   Gomoris One Step Trichrome [Class II]
   Verhoeff's Elastin Stain for Elastic Fibers [Class IV]
   Movat's Pentachrome [Class IV]
   Safranin O for Cartilage [Class II]
   Picrosirius Red For Cardiac Muscle [Class II]
   Trichrome Masson [Class III]
   Herovici [Class III]
   TRAP Stain for Osteoclasts [Class IV]
 
  Histological Staining - Cytoplasmic Granules
   FontanaMasson Silver Method for Argentaffin Granules [Class IV]
   Gomoris Method for Pancreatic Islet Cells [Class II]
   Toluidine Blue for Mast Cells [Class I]
   Chloroacetate Esterase [CAE] [Class III]
 
  Histological Staining - Fats and Lipids
   Oil Red O [Class III]
   Sudan Black B [Class III]
   Pearse's Method for Phospholipids [Class IV]
 
  Histological Staining - Hematologic and Nuclear Elements
   MayGruneald Giemsa Method [Class III]
   Cresyl Violet- Vogt's [Class II]
   Carstairs' Method for Fibrin & Platelets [Class II]
 
  Histological Staining - Nerve Cells and Fibers
   Bielschowski's Silver Method [Class IV]
   PTAH for CNS Tissue [Class IV]
   Luxol Fast Blue [Class III]
   Vanadium Acid Fushin Toluidine Blue [Class III]
 
  Histological Staining - Pigments and Minerals
   VonKossa's Method for Calcium [Class IV]
   Quinalizarian for Calcium [Class II]
   Prussian Blue for Iron [Class I]
 
  Histological Staining- Double Hx Stains
   Luxol Fast Blue [Class III] / H&E
   Periodic AcidSchiff's [PAS] [Class I] / Alcian Blue [Class I]
 
 
Miscellaneous
  Shipping and Handling [RHP]
   Handling Charge
   FedEx Charges [For Pathologist Review]
 
  Supplies [RHP]
   Slide Storage Boxes [25 slide capacity]
   Slide Storage Boxes [100 slide capacity]
   RNAse Free
 
 
Supplementary Support
  Technical Support
   Technical Support [RHP]
 
  Professional Support
   Pathologist- Rodent Histopathology Core [Pre-approval required]