Service Root: Histopathology Services


'Offered by Core' Key
BWH DF/HCC Pathology Core
HMS Mouse Engineering
Pathology Specimen Locator [DF/HCC]
Rodent Histopathology [DF/HCC]
Specialized Histopathology Services - Longwood
Specialized Histopathology Services - Longwood (CLIA)
Specialized Histopathology Services - Longwood [DF/HCC]
Specialized Histopathology Services - MGH
Specialized Histopathology Services - MGH [DF/HCC]
Tissue Microarray & Imaging
Tissue Microarray & Imaging [DF/HCC]




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Conventional Histology Services
     Necropsy [Gross exam/harvest only. Does not include cassetting, processing, embedding, & sectioning]
         Tissue Trimming and Cassetting - Paraffin
         Processing- Paraffin
         Embedding- Paraffin
         Creation of Frozen Block in OCT
       *Std Sectioning- Paraffin [1st Slide]
         *Custom Step Sectioning- Paraffin [1st Section]
         *Serial Sectioning- Paraffin [1st Slide]
         *Std Sectioning- Frozen [1st Slide]
         *Custom Step Sectioning- Frozen [1st Section]
         *Serial Sectioning- Frozen [1st Slide]
       *H&E Paraffin
       *H&E Frozen
       Cell Pellet Preparation
     Partial Cell Pellet Preparation
       Non-Decalcified Bone Sectioning - Frozen
     CryoJane Transfer Method Sectioning- Frozen [1st Slide]
     Std Sectioning- Paraffin [Additional Slide] FY15
     Custom Step Sectioning- Paraffin [Additional Section]
     Serial Sectioning- Paraffin [Additional Slide] FY15
     Std Sectioning- Frozen [Additional Slide] FY15
     Custom Step Sectioning- Frozen [Additional Section]
     Serial Sectioning- Frozen [Additional Slide] FY15
       10-50 Micron Scrolls
     CryoJane Transfer Method Sectioning- Frozen [Additional Slide]
         *Routine Step Sectioning- Paraffin [1st Slide]
         *Routine Step Sectioning- Frozen [1st Slide]
         *Routine Step Sectioning- Paraffin [Additional Slide]
         *Routine Step Sectioning- Frozen [Additional Slide]
         H&E Stain [Paraffin & Frozen]
     Paraffin Sectioning- 1st Slide
     Frozen Sectioning- 1st Slide
     Dehydrate & Coverslip Only
     Sectioning [1st Slide] FY16
     Sectioning [Additional Slide] FY16
     Paraffin Slide Dipping
     Frozen Sectioning- Per Section
     Paraffin Sectioning- Per Section
     Paraffin Sectioning- Per Slide
     Frozen Sectioning- Per Slide
     Paraffin Sectioning- Per Slide
     Paraffin Sectioning- Per Section
     Frozen Sectioning- Per Slide
     Frozen Sectioning- Per Section
     Paraffin Sectioning w/ Tape- Per Slide
     Frozen Sectioning w/ Tape- Per Slide
     Jumbosette Embedding- Paraffin
     Jumbosette Processing- Paraffin
     Jumbosette Sectioning- Paraffin
     Sectioning [Paraffin & Frozen]
     Jumbosette [2x3] Tissue Trimming and Cassetting
     Jumbosette [2x3] Processing
     Jumbosette [2x3] Embedding
     Jumbosette [2x3] Sectioning
     Jumbosette [2x3] H&E Stain
     Paraffin Step Sectioning- Per Slide
     Frozen Step Sectioning- Per Slide
     Paraffin Sectioning for Histogel- Per Slide
     Macro Embedding Single Punch
     Preparation of Slides for Single Cell RNA-Seq/Genomics Visium Platform
 
Histological Staining - Apoptosis
     TUNEL Assay- Kit provided by Core
       TUNEL Assay- Kit provided by PI
     TUNEL Assay- Human Tissue Only [Core SOP]
 
Histological Staining - Bacteria, Fungi, and Inclusion Bodies
       Ziehl Neelsen Method for Acid Fast Bacteria [Class I]
       Grocott's Method for Fungi [GMS] [Class IV]
       Brown and Brenn Method for Gram +/ Bacteria [Class III]
       WarthinStarry Method for Spirochetes [Class IV]
 
Histological Staining - Carbohydrate and Mucoproteins
       Congo Red for Amyloid [Class II]
       Crystal Violet for Amyloids [Class II]
         Periodic AcidSchiff's [PAS] [Class I]
       Mayer's Mucicarmine Method for Mucin and Cryptococcus [Class II]
         Alcian Blue for Mucosubstances [Class I]
       Collodial Iron for Acid Mucopolysaccarides [Class II]
     Jones Methemine Silver for Basement Membrane and Kidney Disease [Class IV]
 
Histological Staining - Connective Tissue and Muscle
       Van Gieson's Method for Collagen Fibers [Class I]
       Mallory's Aniline Blue Collagen Stain [Class I]
       Gomoris Aldehyde Fuchsin Method [Class II]
       AyoubShklar Method for Keratin and Prekeratin [Class I]
       Mallory's Phosphotungstic Acid Hematoxylin Method [PTAH] [Class III]
       Wilder's Method for Reticulum [Class III]
       Gomoris One Step Trichrome [Class II]
         Verhoeff's Elastin Stain for Elastic Fibers [Class IV]
       Movat's Pentachrome [Class IV]
       Safranin O for Cartilage [Class II]
         Picrosirius Red For Cardiac Muscle [Class II]
         Trichrome Masson [Class III]
     Herovici [Class III]
     TRAP Stain for Osteoclasts [Class IV]
 
Histological Staining - Cytoplasmic Granules
       FontanaMasson Silver Method for Argentaffin Granules [Class IV]
       Gomoris Method for Pancreatic Islet Cells [Class II]
       Luna's Method for Mast Cells [Class I]
       Toluidine Blue for Mast Cells [Class I]
     Chloroacetate Esterase [CAE] [Class III]
 
Histological Staining - Fats and Lipids
         Oil Red O [Class III]
       Sudan Black B [Class III]
       Pearse's Method for Phospholipids [Class IV]
 
Histological Staining - Hematologic and Nuclear Elements
       MayGruneald Giemsa Method [Class III]
       Nucleic Acid Stain [Class II]
       Cresyl Violet- Vogt's [Class II]
     Carstairs' Method for Fibrin & Platelets [Class II]
 
Histological Staining - Nerve Cells and Fibers
       Bielschowski's Silver Method [Class IV]
       Bodian's Method for Nerve Fibers and Nerve Endings [Class IV]
       PTAH for CNS Tissue [Class IV]
         Luxol Fast Blue [Class III]
       Vanadium Acid Fushin Toluidine Blue [Class III]
 
Histological Staining - Pigments and Minerals
       Hall's Method for Bilirubin [Class I]
         VonKossa's Method for Calcium [Class IV]
       Quinalizarian for Calcium [Class II]
         Prussian Blue for Iron [Class I]
       AFIP Method for Lipofuscin [Class III]
     Alizarin Red [Class IV]
     Rhodanine Stain for Copper [Class III]
 
Eye Pathology [Human and Mouse]
     Gross Examination of Globes [Human Eye]
     Photography [Human Eye]
     Tissue Trimming and Cassetting – Paraffin [Human Eye]
     Processing- Paraffin [Human Eye]
     Embedding- Paraffin [Human Eye]
     Sectioning- Paraffin [Human Eye]- 1st slide
     Sectioning- Paraffin [Human Eye]- Additional slide
     Sectioning- Paraffin [Mouse Eye]- 1st slide
     Sectioning- Paraffin [Mouse Eye]- Additional Slide
     H&E- Paraffin [Human Eye]
 
Histological Staining- Double Hx Stains
     Luxol Fast Blue [Class III] / H&E
     Periodic AcidSchiff's [PAS] [Class I] / Alcian Blue [Class I]