Services Provided by Specialized Histopathology Services - MGH Core


Technical Contact:  
Diana Gluhova : 617-726-5510

Core Location:
Massachusetts General Hospital
East CNY6, 6122 Drop-Off Hrs 8-3:30pm




Catalog Home
View Core Policies
Print Entire Catalog




   
Histopathology Services
  Conventional Histology Services
   Tissue Trimming and Cassetting - Paraffin
   Processing- Paraffin
   Embedding- Paraffin
   Creation of Frozen Block in OCT
   10-50 Micron Scrolls
   H&E Stain [Paraffin & Frozen]
   Frozen Sectioning- Per Section
   Paraffin Sectioning- Per Section
   Paraffin Sectioning- Per Slide
   Frozen Sectioning- Per Slide
   Jumbosette Embedding- Paraffin
   Jumbosette Processing- Paraffin
   Paraffin Step Sectioning- Per Slide
   Frozen Step Sectioning- Per Slide
 
  Histological Staining - Apoptosis
   TUNEL Assay- Kit provided by Core
 
  Histological Staining - Carbohydrate and Mucoproteins
   Periodic AcidSchiff's [PAS] [Class I]
   Alcian Blue for Mucosubstances [Class I]
 
  Histological Staining - Connective Tissue and Muscle
   Verhoeff's Elastin Stain for Elastic Fibers [Class IV]
   Picrosirius Red For Cardiac Muscle [Class II]
   Trichrome Masson [Class III]
 
  Histological Staining - Fats and Lipids
   Oil Red O [Class III]
 
  Histological Staining - Nerve Cells and Fibers
   Luxol Fast Blue [Class III]
 
  Histological Staining - Pigments and Minerals
   VonKossa's Method for Calcium [Class IV]
   Prussian Blue for Iron [Class I]
 
  Eye Pathology [Human and Mouse]
   Gross Examination of Globes [Human Eye]
   Photography [Human Eye]
   Tissue Trimming and Cassetting – Paraffin [Human Eye]
   Processing- Paraffin [Human Eye]
   Embedding- Paraffin [Human Eye]
   Sectioning- Paraffin [Human Eye]- 1st slide
   Sectioning- Paraffin [Human Eye]- Additional slide
   Sectioning- Paraffin [Mouse Eye]- 1st slide
   Sectioning- Paraffin [Mouse Eye]- Additional Slide
   H&E- Paraffin [Human Eye]
 
 
Immunohistochemistry [IHC] Services
  Standardized IHC
   Single IHC Staining- AB provided by core [Manual]
   Double IHC Staining- AB provided by core [Manual]
   Single IHC Staining- AB provided by customer [Manual]
   Double IHC Staining- AB provided by customer [Manual]
   Single IHC Staining- AB provided by core [Automated Ventana]
   Double IHC Staining- AB provided by core [Automated Ventana]
   Single IHC Staining- AB provided by customer [Automated Ventana]
   Double IHC Staining- AB provided by customer [Automated Ventana]
   Each Additional IHC Ab Multi-plex Staining
 
  Antibody Optimization [Abs must be provided by investigator]
   Ab Optimization- Commercial & Novel Antibodies [Automated Ventana Platform]
   Ab Optimization- Initial Double Staining Optimization [Automated Ventana Platform]
   Ab Optimization- Commercial & Novel Antibodies [Manual]
   Ab Optimization- Initial Double Staining Optimization [Manual]
 
 
Equipment Training and Unassisted Use
  Cryostat
   Cryostat Training
   Unassisted Use - Cryostat
 
  Paraffin Microtome
   Paraffin Microtome Training
   Unassisted Use - Paraffin Microtome
 
  Embedding Center
   Embedding Center Training
   Unassisted Use - Embedder Center
 
  Microscopy
   Unassisted Use - Microscope [Only on Core Stained Slides]
 
 
Miscellaneous
  Shipping and Handling [SHM]
   Handling Charge
 
  Supplies and Services [SHM]
   Slide Storage Boxes [5 slide capacity]
   Control Slides [Paraffin]
   Block Panel Storage
   Slide Storage Boxes [25 slide capacity]
   Slide Storage Boxes [100 slide capacity]
 
 
Supplementary Support
  Technical Support
   Technical Support [SHM]
 
  Professional Support
   Pathologist- Specialized Histopathology Services- MGH [Pre-approval required]
 
 
Immunofluorescent Staining
  Immunofluorescent [IF] Staining
   Each Additional IF Ab Multi-plex Staining
   Single IF Staining- AB provided by core [Automated Ventana]
   Double IF Staining- AB provided by core [Automated Ventana]
   Single IF Staining- AB provided by customer [Automated Ventana]
   Double IF Staining- AB provided by customer [Automated Ventana]
   IF Optimization- Commercial & Novel Antibodies [Automated Ventana]
   IF Optimization- Initial Double Staining [Automated Ventana]
   Single IF Staining- AB provided by core [Manual]
   Single IF Staining- AB provided by customer [Manual]