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Histopathology Services
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Conventional Histology Services
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Tissue Trimming and Cassetting - Paraffin
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Processing- Paraffin
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Embedding- Paraffin
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Creation of Frozen Block in OCT
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Cell Pellet Preparation
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10-50 Micron Scrolls
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H&E Stain [Paraffin & Frozen]
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Sectioning [Paraffin & Frozen]
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Macro Embedding Single Punch
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Preparation of Slides for Single Cell RNA-Seq/Genomics Visium Platform
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Histological Staining - Bacteria, Fungi, and Inclusion Bodies
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Ziehl Neelsen Method for Acid Fast Bacteria [Class I]
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Grocott's Method for Fungi [GMS] [Class IV]
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Brown and Brenn Method for Gram +/ Bacteria [Class III]
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WarthinStarry Method for Spirochetes [Class IV]
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Histological Staining - Carbohydrate and Mucoproteins
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Congo Red for Amyloid [Class II]
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Crystal Violet for Amyloids [Class II]
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Periodic AcidSchiff's [PAS] [Class I]
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Mayer's Mucicarmine Method for Mucin and Cryptococcus [Class II]
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Alcian Blue for Mucosubstances [Class I]
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Collodial Iron for Acid Mucopolysaccarides [Class II]
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Jones Methemine Silver for Basement Membrane and Kidney Disease [Class IV]
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Histological Staining - Connective Tissue and Muscle
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Mallory's Aniline Blue Collagen Stain [Class I]
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Mallory's Phosphotungstic Acid Hematoxylin Method [PTAH] [Class III]
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Wilder's Method for Reticulum [Class III]
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Verhoeff's Elastin Stain for Elastic Fibers [Class IV]
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Movat's Pentachrome [Class IV]
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Safranin O for Cartilage [Class II]
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Picrosirius Red For Cardiac Muscle [Class II]
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Trichrome Masson [Class III]
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Histological Staining - Cytoplasmic Granules
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FontanaMasson Silver Method for Argentaffin Granules [Class IV]
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Toluidine Blue for Mast Cells [Class I]
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Histological Staining - Fats and Lipids
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Oil Red O [Class III]
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Sudan Black B [Class III]
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Histological Staining - Hematologic and Nuclear Elements
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MayGruneald Giemsa Method [Class III]
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Cresyl Violet- Vogt's [Class II]
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Histological Staining - Nerve Cells and Fibers
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Bielschowski's Silver Method [Class IV]
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Bodian's Method for Nerve Fibers and Nerve Endings [Class IV]
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PTAH for CNS Tissue [Class IV]
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Luxol Fast Blue [Class III]
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Histological Staining - Pigments and Minerals
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VonKossa's Method for Calcium [Class IV]
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Prussian Blue for Iron [Class I]
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Alizarin Red [Class IV]
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Rhodanine Stain for Copper [Class III]
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Immunohistochemistry [IHC] Services
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Standardized IHC
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Single Staining IHC
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Double Staining IHC
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Single Staining IHC [Ventana Platform]
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Double Staining IHC [Ventana Platform]
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Each Additional IHC Ab Multi-plex Staining
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Amplified Optiview Ventana IHC [Single staining only]
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Antibody Optimization [Abs must be provided by investigator]
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Ab Optimization- Commercial & Novel Antibodies
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Ab Optimization- Initial Double Staining Optimization
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Ab Optimization- Commercial & Novel Antibodies [Ventana Platform]
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Ab Optimization- Initial Double Staining Optimization [Ventana Platform]
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Equipment Training and Unassisted Use
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Fluorescence Microscope
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Unassisted Use- Fluorescence Microscope [EVOS Only]
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Fluorescence Microscope Training [EVOS Only]
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Miscellaneous
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SHL- Supplies
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Slide Storage Boxes [25 slide capacity]
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Slide Storage Boxes [100 slide capacity]
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RNAse Away
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Control Slide: Phospho-Markers
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Signal Amplification
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Handling Charge
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Tall Slide Box, 25 slides [2x3 slides]
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Tall Slide Box, 100 slides [2x3 slides]
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Filoslide 9 Slide Tray- Cardboard
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Microtome Blade Change
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Supplementary Support
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Technical Support
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SHL- Technical Assistance and Consultation
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Professional Support
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SHL- Core Pathologist Support [Pre-approval required]
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SHL- Non-Core Pathologist Support [Pre-approval required]
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In Situ Hybridization [ISH] Services
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SHL- In Situ Hybridization
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CISH Services [reagents provided by customer]
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FISH Services [reagents provided by customer]
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Immunofluorescent Staining
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Immunofluorescent [IF] Staining
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Single IF Staining
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Double IF Staining
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IF Optimization- Commercial & Novel Antibodies
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IF Optimization- Initial Double Staining
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Each Additional IF Ab Multi-plex Staining
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Fluorescent Image Snapshot [EVOS Only]
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Pathology Specimen Services
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SHL- Block/Slide Retrieval & Associated Services
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Block Retrieval- BWH [Not released to investigators]
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Original H&E Slide Retrieval- BWH [Not released to investigators]
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Block Retrieval- BWH Faulkner [Not released to investigators]
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Original H&E Slide Retrieval- BWH Faulkner [Not released to investigators]
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Unstained Slide- BWH Faulkner
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H&E Stained Slide- BWH Faulkner
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Paraffin Scrolls- BWH Faulkner
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Block Retrieval- MGH [Not released to investigators]
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Original H&E Slide Retrieval- MGH [Not released to investigators]
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Unstained Slide- MGH
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H&E Stained Slide- MGH
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Paraffin Scrolls- MGH
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Block Selection by Core Staff
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Block Storage & Management [Large Cohort Studies Only]
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Block Retrieval- BWH Neuropath [Not released to investigators]
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